Nile Red
Nile Red
CAS: 7385-67-3
Molecular Formula: C20H18N2O2
Nile Red - Names and Identifiers
| Name | Nile Red |
| Synonyms | NILE RED Nile Red NILE BLUE A OXAZONE 9-(diethylamino)-5h-benzo[a]phenoxazin-5-on 9-(diethylamino)benzo[a]phenoxazin-5(5H)-one |
| CAS | 7385-67-3 |
| EINECS | 230-966-0 |
| InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
Nile Red - Physico-chemical Properties
| Molecular Formula | C20H18N2O2 |
| Molar Mass | 318.37 |
| Density | 1.1871 (rough estimate) |
| Melting Point | 203-205 °C(lit.) |
| Boling Point | 457.53°C (rough estimate) |
| Solubility | Soluble in water (Partly miscible), and methanol (1 mg/ml). |
| Appearance | Powder |
| Color | Dark red to maroon |
| BRN | 279110 |
| pKa | 4.08±0.20(Predicted) |
| Storage Condition | 2-8°C |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Sensitive | Sensitive to light |
| Refractive Index | 1.5700 (estimate) |
| MDL | MFCD00011639 |
| Physical and Chemical Properties | Bioactive Nile Red (Nile Blue A oxazone, Phenoxazone 9) is a very effective fluorescent dye, which can detect lipid droplets intracellular intracellular lipid droplets in hydrophobic environment. Nile Red can be used for staining of intracellular lipids, hydrophobic waters of proteins, and lysosomal phospholipid inclusions. |
| Use | Use biochemical research. |
Nile Red - Risk and Safety
| Safety Description | 24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 8-10 |
| HS Code | 29349990 |
Nile Red - Upstream Downstream Industry
| Raw Materials | Ethanol Sodium hydroxide Hydrochloric acid Diethyl ether Acetic acid Sodium nitrite Isopropyl alcohol Nickel 3-Diethylaminophenol 1,4-Naphthoquinone 2-Hydroxy-1,4-naphoquinone |
Nile Red - Nature
| EPA chemical information | 5H-Benzo[a]phenoxazin-5-one, 9-(diethylamino)- (7385-67-3) |
Last Update：2024-04-10 22:29:15
Nile Red - Uses and synthesis methods
Target
Target Value
lipid droplet
in vitro studies
Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.
In vivo studies
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.
Last Update：2024-04-10 22:29:15
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Raw Materials for Nile Red